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ACIDITHIOBACILLUS THIOOXIDANS MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Medium for Acidithiobacillus thiooxidans DSM 103717
* Modification: Omit thiosulfate from the medium and add 10.00 g/l elemental sulfur as substrate. For sterilization place the sulfur in screw-capped tubes, add 1-2 drops of distilled water and incubate on 3 successive days for 3 h at 90-100°C in a water bath. Before use, aseptically layer the sulfur onto the surface of autoclaved liquid basal medium.
Growth conditions: 3-7 days
30 °C
Main sol. 71 #
ml
Equipment needed: Autoclave
Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KH2PO4 | 3.00 | g | 3 | 22.045 | |
| MgSO4 x 7 H2O | 0.50 | g | 0.5 | 2.029 | |
| (NH4)2SO4 | 3.00 | g | 3 | 22.703 | |
| CaCl2 x 2 H2O | 0.25 | g | 0.25 | 1.701 | |
| Na2S2O3 x 5 H2O* | 5.00 | g | 5 | 20.146 | |
| Elemental sulfur* | 10.00 | g | - | - | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Prepare the medium without thiosulfate, adjust pH to 4.4 - 4.7 and autoclave at 121°C for 15 min. Sterilize thiosulfate separately by filtration and add after autoclaving. | |||||
| 2 Note: Incubate statically without shaking. | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Defined medium |
| Final pH: | 4.4 - 4.7 |
| Equipment needed: | Autoclave, Filter |
| Edit in Medium builder | |
Molecular composition
* modified
beta
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Elemental sulfur | 10 | - |
| (NH4)2SO4 | 3 | 22.703 |
| KH2PO4 | 3 | 22.045 |
| MgSO4 x 7 H2O | 0.5 | 2.029 |
| CaCl2 x 2 H2O | 0.25 | 1.701 |
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